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ki 67  (OriGene)


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    Structured Review

    OriGene ki 67
    Characterization of patient-derived lung cancer organoids, Related to . (A) Histological and immunohistochemical analysis of patient 3 tumor tissue and corresponding organoids. H&E staining shows the overall tissue morphology. Immunohistochemistry was performed to detect CK7, TTF-1, <t>and</t> <t>Ki-67</t> expression. Scale bars represent 100 µm. (B) Immunofluorescence staining of patient 3 organoids. Organoids were stained for F-actin, Ki67, NapsinA, CK7, and TTF-1. Scale bar represents 100 µm. (C) DNA copy number analysis of four patients-derived organoids using mutation detection. Each point represents a single genomic probe. Red points indicate gains, green points indicate losses, and blue points represent neutral copy number. Chromosomes are indicated along the x-axis.
    Ki 67, supplied by OriGene, used in various techniques. Bioz Stars score: 95/100, based on 67 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/c09+12/Chromogen+Kits+for+Peroxidase/bio_rxiv__64898__2026__04__16__718819-184-11-27
    Average 95 stars, based on 67 article reviews
    ki 67 - by Bioz Stars, 2026-09
    95/100 stars

    Images

    1) Product Images from "Application of a High-Biomimetic Tumor Organoid-CAF Co-Culture Model for the Efficacy Evaluation of CAR-T Drugs"

    Article Title: Application of a High-Biomimetic Tumor Organoid-CAF Co-Culture Model for the Efficacy Evaluation of CAR-T Drugs

    Journal: bioRxiv

    doi: 10.64898/2026.04.16.718819

    Characterization of patient-derived lung cancer organoids, Related to . (A) Histological and immunohistochemical analysis of patient 3 tumor tissue and corresponding organoids. H&E staining shows the overall tissue morphology. Immunohistochemistry was performed to detect CK7, TTF-1, and Ki-67 expression. Scale bars represent 100 µm. (B) Immunofluorescence staining of patient 3 organoids. Organoids were stained for F-actin, Ki67, NapsinA, CK7, and TTF-1. Scale bar represents 100 µm. (C) DNA copy number analysis of four patients-derived organoids using mutation detection. Each point represents a single genomic probe. Red points indicate gains, green points indicate losses, and blue points represent neutral copy number. Chromosomes are indicated along the x-axis.
    Figure Legend Snippet: Characterization of patient-derived lung cancer organoids, Related to . (A) Histological and immunohistochemical analysis of patient 3 tumor tissue and corresponding organoids. H&E staining shows the overall tissue morphology. Immunohistochemistry was performed to detect CK7, TTF-1, and Ki-67 expression. Scale bars represent 100 µm. (B) Immunofluorescence staining of patient 3 organoids. Organoids were stained for F-actin, Ki67, NapsinA, CK7, and TTF-1. Scale bar represents 100 µm. (C) DNA copy number analysis of four patients-derived organoids using mutation detection. Each point represents a single genomic probe. Red points indicate gains, green points indicate losses, and blue points represent neutral copy number. Chromosomes are indicated along the x-axis.

    Techniques Used: Derivative Assay, Immunohistochemical staining, Staining, Immunohistochemistry, Expressing, Immunofluorescence, Mutagenesis

    Histological and Molecular Concordance Analysis of Tumor Tissue and Organoid. (A) Histological and immunohistochemical concordance of tumor tissue and organoids from Patient 1 and Patient 2. H&E staining shows the overall histological structure, while IHC for CK7, TTF-1, and Ki-67 demonstrates the expression of these markers in both tissue and organoid samples. Scale bars represent 100 μm. (B) Fluorescence microscopy images of Patient 6 organoids. DAPI staining (blue) highlights the nuclei, F-actin staining (red) shows the cytoskeleton, and immunofluorescence for NapsinA, CK7, TTF-1, and Ki-67 (green) indicates the expression and localization of these proteins. Scale bars represent 100 μm. (C) Heatmap showing the correlation between gene expression profiles of tumor (T) and organoid (O) samples from five patients. The color scale ranges from blue (low correlation) to red (high correlation), indicating the similarity in gene expression between corresponding tumor and organoid samples. (D) Mutation profile comparison between tumor (T) and organoid (O) samples. (E) DNA copy number variation (CNV) analysis of Patient 1 and Patient 2 between tumor (T) and organoid (O) samples.
    Figure Legend Snippet: Histological and Molecular Concordance Analysis of Tumor Tissue and Organoid. (A) Histological and immunohistochemical concordance of tumor tissue and organoids from Patient 1 and Patient 2. H&E staining shows the overall histological structure, while IHC for CK7, TTF-1, and Ki-67 demonstrates the expression of these markers in both tissue and organoid samples. Scale bars represent 100 μm. (B) Fluorescence microscopy images of Patient 6 organoids. DAPI staining (blue) highlights the nuclei, F-actin staining (red) shows the cytoskeleton, and immunofluorescence for NapsinA, CK7, TTF-1, and Ki-67 (green) indicates the expression and localization of these proteins. Scale bars represent 100 μm. (C) Heatmap showing the correlation between gene expression profiles of tumor (T) and organoid (O) samples from five patients. The color scale ranges from blue (low correlation) to red (high correlation), indicating the similarity in gene expression between corresponding tumor and organoid samples. (D) Mutation profile comparison between tumor (T) and organoid (O) samples. (E) DNA copy number variation (CNV) analysis of Patient 1 and Patient 2 between tumor (T) and organoid (O) samples.

    Techniques Used: Immunohistochemical staining, Staining, Expressing, Fluorescence, Microscopy, Immunofluorescence, Gene Expression, Mutagenesis, Comparison



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    Image Search Results


    Characterization of patient-derived lung cancer organoids, Related to . (A) Histological and immunohistochemical analysis of patient 3 tumor tissue and corresponding organoids. H&E staining shows the overall tissue morphology. Immunohistochemistry was performed to detect CK7, TTF-1, and Ki-67 expression. Scale bars represent 100 µm. (B) Immunofluorescence staining of patient 3 organoids. Organoids were stained for F-actin, Ki67, NapsinA, CK7, and TTF-1. Scale bar represents 100 µm. (C) DNA copy number analysis of four patients-derived organoids using mutation detection. Each point represents a single genomic probe. Red points indicate gains, green points indicate losses, and blue points represent neutral copy number. Chromosomes are indicated along the x-axis.

    Journal: bioRxiv

    Article Title: Application of a High-Biomimetic Tumor Organoid-CAF Co-Culture Model for the Efficacy Evaluation of CAR-T Drugs

    doi: 10.64898/2026.04.16.718819

    Figure Lengend Snippet: Characterization of patient-derived lung cancer organoids, Related to . (A) Histological and immunohistochemical analysis of patient 3 tumor tissue and corresponding organoids. H&E staining shows the overall tissue morphology. Immunohistochemistry was performed to detect CK7, TTF-1, and Ki-67 expression. Scale bars represent 100 µm. (B) Immunofluorescence staining of patient 3 organoids. Organoids were stained for F-actin, Ki67, NapsinA, CK7, and TTF-1. Scale bar represents 100 µm. (C) DNA copy number analysis of four patients-derived organoids using mutation detection. Each point represents a single genomic probe. Red points indicate gains, green points indicate losses, and blue points represent neutral copy number. Chromosomes are indicated along the x-axis.

    Article Snippet: Sections were blocked with 3% H2O2, respectively incubated with CK7, TTF-1, Ki-67 and mesothelin primary antibodies at 4°C overnight, followed by secondary antibodies and DAB substate (C09-12, OriGene).

    Techniques: Derivative Assay, Immunohistochemical staining, Staining, Immunohistochemistry, Expressing, Immunofluorescence, Mutagenesis

    Histological and Molecular Concordance Analysis of Tumor Tissue and Organoid. (A) Histological and immunohistochemical concordance of tumor tissue and organoids from Patient 1 and Patient 2. H&E staining shows the overall histological structure, while IHC for CK7, TTF-1, and Ki-67 demonstrates the expression of these markers in both tissue and organoid samples. Scale bars represent 100 μm. (B) Fluorescence microscopy images of Patient 6 organoids. DAPI staining (blue) highlights the nuclei, F-actin staining (red) shows the cytoskeleton, and immunofluorescence for NapsinA, CK7, TTF-1, and Ki-67 (green) indicates the expression and localization of these proteins. Scale bars represent 100 μm. (C) Heatmap showing the correlation between gene expression profiles of tumor (T) and organoid (O) samples from five patients. The color scale ranges from blue (low correlation) to red (high correlation), indicating the similarity in gene expression between corresponding tumor and organoid samples. (D) Mutation profile comparison between tumor (T) and organoid (O) samples. (E) DNA copy number variation (CNV) analysis of Patient 1 and Patient 2 between tumor (T) and organoid (O) samples.

    Journal: bioRxiv

    Article Title: Application of a High-Biomimetic Tumor Organoid-CAF Co-Culture Model for the Efficacy Evaluation of CAR-T Drugs

    doi: 10.64898/2026.04.16.718819

    Figure Lengend Snippet: Histological and Molecular Concordance Analysis of Tumor Tissue and Organoid. (A) Histological and immunohistochemical concordance of tumor tissue and organoids from Patient 1 and Patient 2. H&E staining shows the overall histological structure, while IHC for CK7, TTF-1, and Ki-67 demonstrates the expression of these markers in both tissue and organoid samples. Scale bars represent 100 μm. (B) Fluorescence microscopy images of Patient 6 organoids. DAPI staining (blue) highlights the nuclei, F-actin staining (red) shows the cytoskeleton, and immunofluorescence for NapsinA, CK7, TTF-1, and Ki-67 (green) indicates the expression and localization of these proteins. Scale bars represent 100 μm. (C) Heatmap showing the correlation between gene expression profiles of tumor (T) and organoid (O) samples from five patients. The color scale ranges from blue (low correlation) to red (high correlation), indicating the similarity in gene expression between corresponding tumor and organoid samples. (D) Mutation profile comparison between tumor (T) and organoid (O) samples. (E) DNA copy number variation (CNV) analysis of Patient 1 and Patient 2 between tumor (T) and organoid (O) samples.

    Article Snippet: Sections were blocked with 3% H2O2, respectively incubated with CK7, TTF-1, Ki-67 and mesothelin primary antibodies at 4°C overnight, followed by secondary antibodies and DAB substate (C09-12, OriGene).

    Techniques: Immunohistochemical staining, Staining, Expressing, Fluorescence, Microscopy, Immunofluorescence, Gene Expression, Mutagenesis, Comparison